Characterization of Two Hela Sublines: Tcrc-1 Produces Regan Isoenzyme and Tcrc-2, Non-regan Isoenzyme
نویسندگان
چکیده
HeLa Cells have been found to produce a placental form of alkaline phosphatase (1, 8, 17) which is very similar to the Regan isoenzyme, a carcinoplacental antigen found in cancer patients (7-9) . In order to investigate the nature of gene expression of carcinoplacental proteins, HeLa cells were cloned, and a subline (HeLa TCRC-1), monophenotypic for the Regan isoenzyme was selected . The properties of another line, HeLa TCRC-2 (HeLa 12-2), discovered by Kelly et al . (14) were studied with regard to non-Regan isoenzyme . MATERIALS AND METHODS
منابع مشابه
Evidence for altered gene regulation in HeLa cells returned to culture after growing in immunosuppressed rats.
HeLa TCRC-1, is a monophenotypic cell line for the production of the carcinoplacental Regan isoenzyme of alkaline phosphatase. It has been previously reported that, when this cell line is grown in immunosuppressed rats, there is an alteration of phenotypic expression in that an isoenzyme that is referred to as oncoamnion (FL) becomes the dominant enzyme form. This report describes the isoenzyme...
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عنوان ژورنال:
- The Journal of Cell Biology
دوره 60 شماره
صفحات -
تاریخ انتشار 1974